uggs bestellen Leptospira L-clinical samples Isola

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Leptospira L-clinical samples Isolation and morphological identification

. Leptospirosis patients with acute febrile complications after commonly used penicillin treatment of some patients after infection in the latent or light - period of time, the body has a certain amount of antibody production, and thus the body leptospirosis in the penicillin-specific antibodies and complement factors There may be converted under the action of cell wall deficient L-type and pathogenicity. For L-shape variable, weak anti-permeability, it can not easily be identified and the growth of common culture is not easy, so need special hypertonic medium for L-type to grow well. In this paper,[link widoczny dla zalogowanych], self-made L-medium leptospirosis successfully isolated from clinical specimens leptospirosis L,[link widoczny dla zalogowanych], was found to form soft ball 44 * Chinese Journal of Ecology 1995, Volume 7,[link widoczny dla zalogowanych], No. 5 body,[link widoczny dla zalogowanych], spiral filamentous body and a huge cystic changes in the body such as polymorphism, and the author, and in vitro induced by Penicillium Cable L-shape very similar to leptospirosis. Further transfer L-positive cultures were grown in L-plate culture of Leptospira, appeared filamentous (F)-type colonies, and the general type of bacteria colonies similar to the L-F. . By transmission electron microscopy confirmed that cell wall defects, loss of some or all of the cell wall. To further demonstrate the isolation of the L-type is in fact the L-leptospirosis, we fixed the smear-positive culture, except for Giemsa staining and silver staining, but also focuses on the immune markers were identified, including the indirect immunofluorescence and immunofluorescence staining method (PAP method). Results show that isolation of the L-L-type is really Leptospira. Jubinski 4 blood culture smears reported as pleomorphic Gram-negative bacteria, fluorescent antibody identified as Staphylococcus aureus L-type. Gray (1980) by indirect immunofluorescence method 5O male patients with urethral gonorrhea smear identified 43 cases (86) had gonococcal L-type. Radu by immunofluorescence techniques such as proof of jaundice hemorrhagic leptospirosis infection in mouse rod cells pecking, cytoplasm of Kupffer cells with fluorescent particles. Chiang Mei and other \
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