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 Wysłany: Śro 1:09, 19 Sty 2011 Temat postu: uggs nederland Septic shock rat liver plasma membr |
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Septic shock rat liver plasma membrane calcium ATP enzyme phosphorylation, dephosphorylation changes
torsinratliverduringsepsis. AmJPhysiol1993: 265: R3S5 a R390CHANGESINTHEPHoSPHoRYLATIoNANDDEPHoSPHoRYLATIoNoFCa2 + mTRANSPORTATPaseoFRATLIVERPLAAMEMBRANEDURINGSEPSISGaoAiguo,[link widoczny dla zalogowanych], DongLinwang,[link widoczny dla zalogowanych], WangXueqing,[link widoczny dla zalogowanych], ZhaoDong, LiuNaikui,[link widoczny dla zalogowanych], TangChaoshu, LiuMaosheng (LaboratoryofShockResearch, InstituteofCardiovascularResearch, BeijingMedicalUniversity, Beijing100083) ABSTRACTThepresentstudyinvestigatedthephosphorylationanddephosphorylationofCa a trans-portATPaseofrat1iverplasmamembrane (PM) duringearlyand1atesepticshockbasedOnin. corporationofh a \earlysepsis) and18h (1atesepsis) afterCLP.respectively.KineticanalysisshowedthattheVmaxofCa a inducedCa a ATPasephosphorylationdecreaseddramaticallyduringearlyandlatesepsiswithoutchangesintheKmvalues.SDS-PAGEanalysisdemon.stratedthattheCaz + a A'rPasephosphorylationintermediatewasa107kuprotdn. TheseresultsdemonstratethatthephosphorylationofCa a ATPaseiUratliverplasmamembranewasim-pairedprogressivelywiththeproceedingofsepticphases.Thesefindingswouldcontributeto Hill eunderstanding0fpaIh0physi010gy0falteredhepaticCa leaves homeostasisduringsepsisKEYWORDSsepticshock; hepaticplasmamembrane; phosphorylation; dephosphorylation; SDS-PAGE
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